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验证数据展示

  • WB analysis of mouse lung using 11790-1-AP (same clone as 11790-1-PBS)

    WB analysis of mouse lung using 11790-1-AP (same clone as 11790-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 11790-1-AP (DIO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11790-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 11790-1-AP (same clone as 11790-1-PBS)

    IF Staining of A549 using 11790-1-AP (same clone as 11790-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using DIO1 antibody (11790-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11790-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 11790-1-AP (same clone as 11790-1-PBS)

    FC experiment of HeLa using 11790-1-AP (same clone as 11790-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DIO1 Polyclonal antibody (11790-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 11790-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 11790-1-AP (same clone as 11790-1-PBS)

    IHC staining of human liver cancer using 11790-1-AP (same clone as 11790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11790-1-AP (DIO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11790-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 11790-1-AP (same clone as 11790-1-PBS)

    IHC staining of human liver cancer using 11790-1-AP (same clone as 11790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11790-1-AP (DIO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11790-1-PBS in a different storage buffer formulation.

DIO1 Polyclonal antibody, PBS Only

DIO1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 11790-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

Type 1 DI, TXDI1, ITDI1, EC:1.21.99.4, DIOI

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11790-1-PBS targets DIO1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2410

Product name: Recombinant human DIO1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-125 aa of BC017955

Sequence: GLPQPGLWLKRLWVLLEVAVHVVVGKVLLILFPDRVKRNILAMGEKTGMTRNPHFSHDNWIPTFFSTQYFWFVLKVRWQRLEDTTELGGLAPNCPVVRLSGQRCNIWEFMQGNRPLVLNFGSCT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 deiodinase, iodothyronine, type I
别名 Type 1 DI, TXDI1, ITDI1, EC:1.21.99.4, DIOI
计算分子量 249 aa, 29 kDa
观测分子量 29 kDa, 13 kDa
GenBank蛋白编号BC017955
基因名称 DIO1
Gene ID (NCBI) 1733
RRIDAB_10949197
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49895
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DIO1(Type I iodothyronine deiodinase) is also named as ITDI1, TXDI1,5DI,DIOI and belongs to the iodothyronine deiodinase family.It is a membrane selenoprotein that catalyzes the deiodination of L-thyroxine to the biologically active thyroid hormone 3,3′,5-triiodothyronine.DIO1 is located in liver, kidney, and thyroid,which has both ORD and IRD activities(PMID: 9389495). It has some isoforms produced by alternative splicing with the MW of 29 kDa, 21 kDa, 13 kDa, 23 kDa, 19 kDa, 4 kDa, 13 kDa, 9 kDa and 10 kDa.

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