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验证数据展示

  • WB analysis of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

    WB analysis of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

    WB result of DIAPH3 antibody (14342-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HeLa cells. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

    WB analysis of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14342-1-AP (DIAPH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 14342-1-AP (same clone as 14342-1-PBS)

    WB analysis of HEK-293 using 14342-1-AP (same clone as 14342-1-PBS)

    WB result of DIAPH3 antibody (14342-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIAPH3 transfected HEK-293 cells. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

    IP experiment of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

    IP result of anti-DIAPH3 (IP:14342-1-AP, 4ug; Detection:14342-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

    IF Staining of HeLa using 14342-1-AP (same clone as 14342-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DIAPH3 antibody (14342-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 14342-1-AP (same clone as 14342-1-PBS)

    IHC staining of mouse testis using 14342-1-AP (same clone as 14342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14342-1-AP (DIAPH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DIAPH3 antibody included
  • IHC staining of rat testis using 14342-1-AP (same clone as 14342-1-PBS)

    IHC staining of rat testis using 14342-1-AP (same clone as 14342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 14342-1-AP (DIAPH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14342-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DIAPH3 antibody included
  • Featured Product
  • KD/KO Validated

DIAPH3 Polyclonal antibody, PBS Only

DIAPH3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14342-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

DIAP3, Diaphanous-related formin-3, DRF3, MDia2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DIAPH3 Ready-To-Use IHC Kit. DIAPH3 primary antibody included.

产品信息

14342-1-PBS targets DIAPH3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5656

Product name: Recombinant human DIAPH3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 500-850 aa of BC048963

Sequence: DQEQLNSLSQFKSEYSNLCEPEQFVVVMSNVKRLRPRLSAILFKLQFEEQVNNIKPDIMAVSTACEEIKKSKSFSKLLELVLLMGNYMNAGSRNAQTFGFNLSSLCKLKDTKSADQKTTLLHFLVEICEEKYPDILNFVDDLEPLDKASKVSVETLEKNLRQMGRQLQQLEKELETFPPPEDLHDKFVTKMSRFVISAKEQYETLSKLHENMEKLYQSIIGYYAIDVKKVSVEDFLTDLNNFRTTFMQAIKENIKKREAEEKEKRVRIAKELAERERLERQQKKKRLLEMKTEGDETGVMDNLLEALQSGAAFRDRRKRTPMPKDVRQSLSPMSQRPVLKVCNHGNKPYL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 diaphanous homolog 3 (Drosophila)
别名 DIAP3, Diaphanous-related formin-3, DRF3, MDia2
计算分子量 137 kDa
观测分子量 125-137 kDa
GenBank蛋白编号BC048963
基因名称 DIAPH3
Gene ID (NCBI) 81624
RRIDAB_2092930
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NSV4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DIAPH3 is also refered as diaphanous-related formin-3 which binds to GTP-bound form of Rho and to profilin, acts in a Rho-dependent manner to recruit profilin to the membrane. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. Seven isoforms of DIAPH3 exist due to the alternative splicing. Recently it has been reported that dimerization and actin assembly activity of DIAPH3 isoforms 1 and 7 are essential for induction of specific cell protrusions. It may propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility.

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