验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, U2OS cells |
| Positive IHC detected in | Hepatocellular carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
RMX00088 targets DHRS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Multi-Recombinant |
| 产品类型 | Antibody |
| 全称 | dehydrogenase/reductase (SDR family) member 2 |
| 别名 | Dehydrogenase/reductase SDR family member 2, mitochondrial, Dicarbonyl reductase HEP27, EC:1.1.1.-, HEP27, Protein D |
| 计算分子量 | 300 aa, 32 kDa |
| 观测分子量 | 27-30 kDa |
| GenBank蛋白编号 | BC007339 |
| 基因名称 | DHRS2 |
| Gene ID (NCBI) | 10202 |
| RRID | AB_3750137 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | N/A |
| UNIPROT ID | Q13268 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DHRS2(Dehydrogenase/reductase SDR family member 2) is also named as dicarbonyl reductase HEP27, Protein D and belongs to the short-chain dehydrogenases/reductases (SDR) family. It displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates and there is no reductase activity displayed in vitro with steroids, retinoids and sugars as substrates. This protein also may inhibit cell replication. It has 2 isoforms produced by alternative splicing with the molecular weight of 27 kDa and 29 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DHRS2 antibody RMX00088 | Download protocol |
| IHC protocol for DHRS2 antibody RMX00088 | Download protocol |
| IF protocol for DHRS2 antibody RMX00088 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
