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验证数据展示

  • WB analysis using 27965-1-AP (same clone as 27965-1-PBS)

    WB analysis using 27965-1-AP (same clone as 27965-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27965-1-AP (DHFRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27965-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 27965-1-AP (same clone as 27965-1-PBS)

    IHC staining of mouse heart using 27965-1-AP (same clone as 27965-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27965-1-AP (DHFRL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27965-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 27965-1-AP (same clone as 27965-1-PBS)

    IHC staining of mouse heart using 27965-1-AP (same clone as 27965-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27965-1-AP (DHFRL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27965-1-PBS in a different storage buffer formulation.

DHFRL1 Polyclonal antibody, PBS Only

DHFRL1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27965-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27965-1-PBS targets DHFRL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag10537

Product name: Recombinant human DHFRL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-187 aa of BC063379

Sequence: MFLLLNCIVAVSQNMGIGKNGDLPRPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLARSLDDALKLTERPELANKVDMIWIVGGSSVYKEAMNHLGHLKLFVTRIMQDFESDTFFSEIDLEKYKLLPEYPGILSDVQEGKHIKYKFEVCEKDD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dihydrofolate reductase-like 1
别名
计算分子量 187 aa, 22 kDa
观测分子量 25 kDa
GenBank蛋白编号BC063379
基因名称 DHFRL1
Gene ID (NCBI) 200895
RRIDAB_3086015
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86XF0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DHFRL1, also known as DHFR2, is a key enzyme in folate metabolism. DHFRL1 is a dihydrofolate reductase, that catalyzes the NADPH-dependent reduction of dihydrofolate to the biologically active form, tetrahydrofolate (PMID: 21876184).

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