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验证数据展示

  • WB analysis using 27965-1-AP

    WB analysis using 27965-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27965-1-AP (DHFRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 27965-1-AP

    IHC staining of mouse heart using 27965-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27965-1-AP (DHFRL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 27965-1-AP

    IHC staining of mouse heart using 27965-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27965-1-AP (DHFRL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DHFRL1 Polyclonal antibody

DHFRL1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27965-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

DHFR2, DHFRL1, DHFRP4, Dihydrofolate reductase 2, mitochondrial, dihydrofolate reductase like 1, FLJ16119

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27965-1-AP targets DHFRL1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原

CatNo: Ag10537

Product name: Recombinant human DHFRL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-187 aa of BC063379

Sequence: MFLLLNCIVAVSQNMGIGKNGDLPRPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLARSLDDALKLTERPELANKVDMIWIVGGSSVYKEAMNHLGHLKLFVTRIMQDFESDTFFSEIDLEKYKLLPEYPGILSDVQEGKHIKYKFEVCEKDD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dihydrofolate reductase-like 1
别名 DHFR2, DHFRL1, DHFRP4, Dihydrofolate reductase 2, mitochondrial, dihydrofolate reductase like 1, FLJ16119
计算分子量 187 aa, 22 kDa
观测分子量 25 kDa
GenBank蛋白编号BC063379
基因名称 DHFRL1
Gene ID (NCBI) 200895
RRIDAB_3086015
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86XF0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHFRL1, also known as DHFR2, is a key enzyme in folate metabolism. DHFRL1 is a dihydrofolate reductase, that catalyzes the NADPH-dependent reduction of dihydrofolate to the biologically active form, tetrahydrofolate (PMID: 21876184).

实验方案

Product Specific Protocols
IHC protocol for DHFRL1 antibody 27965-1-APDownload protocol
WB protocol for DHFRL1 antibody 27965-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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