验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, Jurkat cells |
Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
27965-1-AP targets DHFRL1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | Human, mouse |
免疫原 | DHFRL1 fusion protein Ag10537 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | dihydrofolate reductase-like 1 |
别名 | DHFR2, DHFRL1, DHFRP4, Dihydrofolate reductase 2, mitochondrial, dihydrofolate reductase like 1, FLJ16119 |
计算分子量 | 187 aa, 22 kDa |
观测分子量 | 25 kDa |
GenBank蛋白编号 | BC063379 |
基因名称 | DHFRL1 |
Gene ID (NCBI) | 200895 |
RRID | AB_3086015 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q86XF0 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DHFRL1, also known as DHFR2, is a key enzyme in folate metabolism. DHFRL1 is a dihydrofolate reductase, that catalyzes the NADPH-dependent reduction of dihydrofolate to the biologically active form, tetrahydrofolate (PMID: 21876184).
实验方案
Product Specific Protocols | |
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WB protocol for DHFRL1 antibody 27965-1-AP | Download protocol |
IHC protocol for DHFRL1 antibody 27965-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |