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DHFR Recombinant antibody

DHFR Recombinant Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

7P5

Cat No : 81021-1-RR

Print datasheet

Synonyms

DHFR, DHFRP1, dihydrofolate reductase

验证数据展示

  • WB analysis using 81021-1-RR

    WB analysis using 81021-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81021-1-RR (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 81021-1-RR

    WB analysis using 81021-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81021-1-RR (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 81021-1-RR

    IHC staining of human liver cancer using 81021-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81021-1-RR (DHFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 81021-1-RR

    IHC staining of human liver cancer using 81021-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81021-1-RR (DHFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, Jurkat cells, HEK-293 cells, MOLT-4 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81021-1-RR targets DHFR in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human
Immunogen DHFR fusion protein Ag7331 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name dihydrofolate reductase
Synonyms DHFR, DHFRP1, dihydrofolate reductase
Calculated Molecular Weight 21 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC000192
Gene Symbol DHFR
Gene ID (NCBI) 1719
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00374
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).

实验方案

Product Specific Protocols
WB protocol for DHFR antibody 81021-1-RRDownload protocol
IHC protocol for DHFR antibody 81021-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols