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Species Reactivity
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验证数据展示

  • WB analysis using 81021-1-RR (same clone as 81021-1-PBS)

    WB analysis using 81021-1-RR (same clone as 81021-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81021-1-RR (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 81021-1-RR (same clone as 81021-1-PBS)

    WB analysis of HeLa using 81021-1-RR (same clone as 81021-1-PBS)

    WB result of DHFR antibody (81021-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHFR transfected HeLa cells. This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • WB analysis using 81021-1-RR (same clone as 81021-1-PBS)

    WB analysis using 81021-1-RR (same clone as 81021-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81021-1-RR (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 81021-1-RR (same clone as 81021-1-PBS)

    IHC staining of human liver cancer using 81021-1-RR (same clone as 81021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81021-1-RR (DHFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 81021-1-RR (same clone as 81021-1-PBS)

    IHC staining of human liver cancer using 81021-1-RR (same clone as 81021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81021-1-RR (DHFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81021-1-RR

    Affinity and Kinetic Characterization of 81021-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81021-1-RR against Human DHFR were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证
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DHFR Recombinant monoclonal antibody, PBS Only

DHFR Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 81021-1-PBS

产品说明书

CloneNo. 7P5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

EC:1.5.1.3, DHFRP1, 7P5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

81021-1-PBS targets DHFR in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7331

Product name: Recombinant human DHFR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-187 aa of BC000192

Sequence: MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 dihydrofolate reductase
别名 EC:1.5.1.3, DHFRP1, 7P5
计算分子量 21 kDa
观测分子量21 kDa
GenBank蛋白编号BC000192
基因名称 DHFR
Gene ID (NCBI) 1719
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP00374
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).