• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68274-1-Ig (same clone as 68274-1-PBS)

    WB analysis using 68274-1-Ig (same clone as 68274-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68274-1-Ig (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68274-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 68274-1-Ig (same clone as 68274-1-PBS)

    WB analysis of NIH/3T3 using 68274-1-Ig (same clone as 68274-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68274-1-Ig (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68274-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 68274-1-Ig (same clone as 68274-1-PBS)

    FC experiment of MCF-7 using 68274-1-Ig (same clone as 68274-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug DHFR Monoclonal antibody (68274-1-Ig, Clone:4C11E9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68274-1-PBS in a different storage buffer formulation.

DHFR Monoclonal antibody, PBS Only

DHFR Monoclonal Antibody for Indirect ELISA, WB
Cat No. 68274-1-PBS

产品说明书

CloneNo. 4C11E9

宿主/亚型

Mouse / IgG1

种属反应性

mouse, human

应用

Indirect ELISA, WB

别名

EC:1.5.1.3, DHFRP1, 4C11E9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

68274-1-PBS targets DHFR in Indirect ELISA, WB applications and shows reactivity with mouse, human samples.

经测试应用 Indirect ELISA, WB Application Description
经测试反应性 mouse, human
免疫原

CatNo: Ag7318

Product name: Recombinant human DHFR protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-187 aa of BC000192

Sequence: MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dihydrofolate reductase
别名 EC:1.5.1.3, DHFRP1, 4C11E9
计算分子量 21 kDa
观测分子量21 kDa
GenBank蛋白编号BC000192
基因名称 DHFR
Gene ID (NCBI) 1719
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP00374
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).