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验证数据展示

  • WB analysis using 68274-1-Ig

    WB analysis using 68274-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68274-1-Ig (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of NIH/3T3 using 68274-1-Ig

    WB analysis of NIH/3T3 using 68274-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68274-1-Ig (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 68274-1-Ig

    FC experiment of MCF-7 using 68274-1-Ig

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug DHFR Monoclonal antibody (68274-1-Ig, Clone:4C11E9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

DHFR Monoclonal antibody

DHFR Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 68274-1-Ig

产品说明书

CloneNo. 4C11E9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

EC:1.5.1.3, DHFRP1, 4C11E9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68274-1-Ig targets DHFR in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag7318

Product name: Recombinant human DHFR protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-187 aa of BC000192

Sequence: MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dihydrofolate reductase
别名 EC:1.5.1.3, DHFRP1, 4C11E9
计算分子量 21 kDa
观测分子量21 kDa
GenBank蛋白编号BC000192
基因名称 DHFR
Gene ID (NCBI) 1719
RRIDAB_2935357
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP00374
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).

实验方案

Product Specific Protocols
WB protocol for DHFR antibody 68274-1-IgDownload protocol
FC protocol for DHFR antibody 68274-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols