• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DGKA Polyclonal antibody

DGKA Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-11547

Print datasheet

Synonyms

80 kDa diacylglycerol kinase, DAG kinase alpha, DAGK, DAGK1, DGK alpha, DGKA, Diacylglycerol kinase alpha, Diglyceride kinase alpha

验证数据展示

  • IF Staining of Jurkat using CL488-11547

    IF Staining of Jurkat using CL488-11547

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 DGKA antibody (CL488-11547) at dilution of 1:200.

  • FC experiment of HeLa using CL488-11547

    FC experiment of HeLa using CL488-11547

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human DGKA (CL488-11547) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inJurkat cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 11547-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11547 targets DGKA in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat
Immunogen DGKA fusion protein Ag2075 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name diacylglycerol kinase, alpha 80kDa
Synonyms 80 kDa diacylglycerol kinase, DAG kinase alpha, DAGK, DAGK1, DGK alpha, DGKA, Diacylglycerol kinase alpha, Diglyceride kinase alpha
Calculated Molecular Weight 735 aa, 83 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC023523
Gene Symbol DGKA
Gene ID (NCBI) 1606
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23743
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DGKA(Diacylglycerol kinase alpha) is also named as DAGK, DAGK1 and belongs to the eukaryotic diacylglycerol kinase family,which is suggested to attenuate diacylglycerol-induced cell responses through the phosphorylation of this second messenger to phosphatidic acid.DGKA regulates the secretion of lethal exosomes bearing Fas ligand during activation-induced cell death of T lymphocytes.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DGKA antibody CL488-11547Download protocol
FC protocol for CL Plus 488 DGKA antibody CL488-11547Download protocol
Standard Protocols
Click here to view our Standard Protocols