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验证数据展示

  • WB analysis using CL488-67731

    WB analysis using CL488-67731

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-67731 (DFNA5/GSDME antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of SH-SY5Y using CL488-67731

    FC experiment of SH-SY5Y using CL488-67731

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human DFNA5/GSDME (CL488-67731, Clone:1D9C3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated DFNA5/GSDME Monoclonal antibody

DFNA5/GSDME Monoclonal Antibody for WB, FC (Intra)
Cat No. CL488-67731

产品说明书

CloneNo. 1D9C3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, FC (Intra)

别名

DFNA5, Gasdermin E, Gasdermin-E, GSDME, 1D9C3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy GSDME/DFNA5 Ready-To-Use IHC Kit. GSDME/DFNA5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67731-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67731 targets DFNA5/GSDME in WB, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 DFNA5/GSDME fusion protein Ag30514 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 deafness, autosomal dominant 5
别名 DFNA5, Gasdermin E, Gasdermin-E, GSDME, 1D9C3
计算分子量 496 aa, 55 kDa
观测分子量50-55 kDa
GenBank蛋白编号BC019689
基因名称 DFNA5
Gene ID (NCBI) 1687
RRIDAB_3084376
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO60443
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 DFNA5/GSDME antibody CL488-67731Download protocol
FC protocol for CL Plus 488 DFNA5/GSDME antibody CL488-67731Download protocol
Standard Protocols
Click here to view our Standard Protocols