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Desmin Polyclonal antibody

Desmin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 22205-1-AP

Print datasheet

Synonyms

Cell and organelle markers, CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin

验证数据展示

  • WB analysis of mouse heart using 22205-1-AP

    WB analysis of mouse heart using 22205-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 22205-1-AP (Desmin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 22205-1-AP

    IHC staining of human heart using 22205-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with Desmin antibody included
  • IHC staining of human heart using 22205-1-AP

    IHC staining of human heart using 22205-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22205-1-AP (Desmin antibody at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with Desmin antibody included

经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Desmin Ready-To-Use IHC Kit. Desmin primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22205-1-AP targets Desmin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen Desmin fusion protein Ag17525 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name desmin
Synonyms Cell and organelle markers, CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin
Calculated Molecular Weight 470 aa, 54 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC032116
Gene Symbol DES
Gene ID (NCBI) 1674
RRIDAB_2879027
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17661
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

实验方案

Product Specific Protocols
WB protocol for Desmin antibody 22205-1-APDownload protocol
IHC protocol for Desmin antibody 22205-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols