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CoraLite® Plus 488-conjugated DEPDC6/Deptor Polyclonal antibody

DEPDC6/Deptor Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-20985

Print datasheet

Synonyms

DEP domain containing 6, DEP.6, DEPDC6, Deptor, DKFZp564B1778, FLJ12341, FLJ12428, FLJ13854

验证数据展示

  • IF Staining of HepG2 using CL488-20985

    IF Staining of HepG2 using CL488-20985

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 DEPDC6/Deptor antibody (CL488-20985) at dilution of 1:400.

经过测试的应用

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 20985-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-20985 targets DEPDC6/Deptor in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEP domain containing 6
Synonyms DEP domain containing 6, DEP.6, DEPDC6, Deptor, DKFZp564B1778, FLJ12341, FLJ12428, FLJ13854
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberNM_022783
Gene Symbol DEPDC6
Gene ID (NCBI) 64798
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TB45
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DEPDC6, also named as DEPTOR, was identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. It negative regulator of the mTORC1 and mTORC2 signaling pathways. DEPDC6 inhibits the kinase activity of both complexes. The antibody is specific to DEPDC6.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DEPDC6/Deptor antibody CL488-20985Download protocol
Standard Protocols
Click here to view our Standard Protocols