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验证数据展示

  • WB analysis using 33113-1-AP (same clone as 33113-1-PBS)

    WB analysis using 33113-1-AP (same clone as 33113-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33113-1-AP (DENND2D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 33113-1-AP (same clone as 33113-1-PBS)

    IHC staining of human ovary tumor using 33113-1-AP (same clone as 33113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 33113-1-AP (DENND2D antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 33113-1-AP (same clone as 33113-1-PBS)

    IHC staining of human ovary tumor using 33113-1-AP (same clone as 33113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 33113-1-AP (DENND2D antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 33113-1-AP (same clone as 33113-1-PBS)

    IHC staining of human stomach cancer using 33113-1-AP (same clone as 33113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 33113-1-AP (DENND2D antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 33113-1-AP (same clone as 33113-1-PBS)

    IF Staining of A431 using 33113-1-AP (same clone as 33113-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DENND2D antibody (33113-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 33113-1-PBS in a different storage buffer formulation.

DENND2D Polyclonal antibody, PBS Only

DENND2D Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 33113-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DENN Domain Containing 2D , DENN domain-containing protein 2D

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

33113-1-PBS targets DENND2D in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag36381

Product name: Recombinant human DENND2D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 305-471 aa of BC004557

Sequence: FMVGVQMRFQQEVMDSPMEEVLLVNLCEGTFLMSVGDEKDILPPKLQDDILDSLGQGINELKTAEQINEHVSGPFVQFFVKIVGHYASYIKREANGQGHFQERSFCKALTSKTNRRFVKKFVKTQLFSLFIQEAEKSKNPPAGYFQQKILEYEEQKKQKKPREKTVK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DENN/MADD domain containing 2D
别名 DENN Domain Containing 2D , DENN domain-containing protein 2D
观测分子量48-50 kDa
GenBank蛋白编号BC004557
基因名称 DENND2D
Gene ID (NCBI) 79961
RRIDAB_3742860
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9H6A0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DENND2D (DENN domain-containing protein 2D) is a protein containing a DENN domain and belongs to the family of membrane transport proteins, participating in intracellular membrane transport and signal transduction processes. The DENND2D protein comprises several conserved domains, such as the uDENN, DENN, and dDENN domains, which are widely present in various signaling proteins. In cancer research, the expression and methylation status of DENND2D hold significant prognostic value. DENND2D may serve as a novel tumor biomarker for predicting cancer progression and recurrence.

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