验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, MDA-MB-231 cells, MOLT-4 cells, SK-BR-3 cells |
| Positive IHC detected in | human ovary tumor tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33113-1-AP targets DENND2D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag36381 Product name: Recombinant human DENND2D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 305-471 aa of BC004557 Sequence: FMVGVQMRFQQEVMDSPMEEVLLVNLCEGTFLMSVGDEKDILPPKLQDDILDSLGQGINELKTAEQINEHVSGPFVQFFVKIVGHYASYIKREANGQGHFQERSFCKALTSKTNRRFVKKFVKTQLFSLFIQEAEKSKNPPAGYFQQKILEYEEQKKQKKPREKTVK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | DENN/MADD domain containing 2D |
| 别名 | DENN Domain Containing 2D , DENN domain-containing protein 2D |
| 观测分子量 | 48-50 kDa |
| GenBank蛋白编号 | BC004557 |
| 基因名称 | DENND2D |
| Gene ID (NCBI) | 79961 |
| RRID | AB_3742860 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9H6A0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DENND2D (DENN domain-containing protein 2D) is a protein containing a DENN domain and belongs to the family of membrane transport proteins, participating in intracellular membrane transport and signal transduction processes. The DENND2D protein comprises several conserved domains, such as the uDENN, DENN, and dDENN domains, which are widely present in various signaling proteins. In cancer research, the expression and methylation status of DENND2D hold significant prognostic value. DENND2D may serve as a novel tumor biomarker for predicting cancer progression and recurrence.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DENND2D antibody 33113-1-AP | Download protocol |
| IHC protocol for DENND2D antibody 33113-1-AP | Download protocol |
| WB protocol for DENND2D antibody 33113-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
