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验证数据展示

  • WB analysis using 32948-1-AP (same clone as 32948-1-PBS)

    WB analysis using 32948-1-AP (same clone as 32948-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 32948-1-AP (DDX59 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32948-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 32948-1-AP (same clone as 32948-1-PBS)

    IHC staining of human breast cancer using 32948-1-AP (same clone as 32948-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 32948-1-AP (DDX59 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32948-1-PBS in a different storage buffer formulation.

DDX59 Polyclonal antibody, PBS Only

DDX59 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 32948-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

DEAD box protein 59, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX59, Zinc finger HIT domain-containing protein 5, ZNHIT5

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

32948-1-PBS targets DDX59 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag37657

Product name: Recombinant human DDX59 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 335-619 aa of BC041801

Sequence: DIIKQSSVELCGVKIVVVDEADTMLKMGFQQQVLDILENIPNDCQTILVSATIPTSIEQLASQLLHNPVRIITGEKNLPCANVRQIILWVEDPAKKKKLFEILNDKKLFKPPVLVFVDCKLGADLLSEAVQKITGLKRISIHSEKSQIERKNILKGLLEGDYEVVVSTGVLGRGLDLISVRLVVNFDMPSSMDEYVHQIGRVGRLGQNGTAITFINNNSKRLFWDIAKRVKPTGSILPPQLLNSPYLHDQKRKEQQKDKQTQNDLVTGANLMDIIRKHDKSNSQK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 59
别名 DEAD box protein 59, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX59, Zinc finger HIT domain-containing protein 5, ZNHIT5
计算分子量 619 aa, 69 kDa
观测分子量75 kDa
GenBank蛋白编号BC041801
基因名称 DDX59
Gene ID (NCBI) 83479
RRIDAB_3742802
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ5T1V6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DEAD box proteins are multifunctional proteins involved in every aspect in RNA metabolism and have essential roles in many cellular activities. DDX59 is overexpressed in lung adenocarcinoma. DDX59 knockdown reduced cell proliferation, anchorage-independent cell growth, and caused reduction of tumor formation in immunocompromised mice. In multiple lung cancer cells, DDX59 knockdown inhibits DNA synthesis; wild-type DDX59 but not helicase-defective mutant of DDX59 enhances DNA synthesis. DDX59 knockdown caused reduction of MCM protein levels, decreased the loading of MCM ring protein onto chromatin, and therefore inhibited DNA replication. DDX59 has an important role in lung cancer development through promoting DNA replication (PMID: 28090355).

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