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CoraLite® Plus 488-conjugated DDX4,VASA Polyclonal antibody

DDX4,VASA Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-51042

Print datasheet

Synonyms

DDX4, DEAD box protein 4, VASA, Vasa homolog

验证数据展示

  • IF Staining of mouse testis using CL488-51042

    IF Staining of mouse testis using CL488-51042

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 DDX4,VASA antibody (CL488-51042) at dilution of 1:200.

经过测试的应用

Positive IF detected inmouse testis tissue
For other applications, we recommend the unconjugated version of this antibody, 51042-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-51042 targets DDX4,VASA in IF applications and shows reactivity with human, mouse samples.

Tested Applications IF-P
Tested Reactivity human, mouse
Immunogen DDX4,VASA fusion protein Ag0447 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Synonyms DDX4, DEAD box protein 4, VASA, Vasa homolog
Calculated Molecular Weight690aa,76 kDa; 724aa,79 kDa
Observed Molecular Weight 79 kDa
GenBank Accession NumberBC047455
Gene Symbol DDX4
Gene ID (NCBI) 54514
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQI0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DDX4,VASA antibody CL488-51042Download protocol
Standard Protocols
Click here to view our Standard Protocols