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DDX17 Polyclonal antibody
DDX17 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, HEK-293T cells, HEK-293 cells, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Hela cells, HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
19910-1-AP targets DDX17 in WB, IP, IF, PLA, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF, PLA, IHC, CoIP, ChIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | DDX17 fusion protein Ag13723 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 |
Synonyms | DDX17, DEAD box protein 17, DEAD box protein p72, P72, RH70, RNA dependent helicase p72 |
Calculated Molecular Weight | 729 aa, 80 kDa |
Observed Molecular Weight | 72-80 kDa |
GenBank Accession Number | BC000595 |
Gene Symbol | DDX17 |
Gene ID (NCBI) | 10521 |
RRID | AB_10667004 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q92841 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for DDX17 antibody 19910-1-AP | Download protocol |
IHC protocol for DDX17 antibody 19910-1-AP | Download protocol |
IF protocol for DDX17 antibody 19910-1-AP | Download protocol |
IP protocol for DDX17 antibody 19910-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Intragenic recruitment of NF-κB drives splicing modifications upon activation by the oncogene Tax of HTLV-1. | ||
Nucleic Acids Res The RNA helicase DDX17 controls the transcriptional activity of REST and the expression of proneural microRNAs in neuronal differentiation. | ||
Nucleic Acids Res RNA helicase-dependent gene looping impacts messenger RNA processing
| ||
PLoS Pathog Hepatitis B virus Core protein nuclear interactome identifies SRSF10 as a host RNA-binding protein restricting HBV RNA production. | ||
J Biol Chem RNA Polymerase III Regulates Cytosolic RNA:DNA Hybrids and Intracellular MicroRNA Expression. | ||
iScience Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells |