• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67991-1-Ig (same clone as 67991-1-PBS)

    WB analysis using 67991-1-Ig (same clone as 67991-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67991-1-Ig (DDX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

  • WB analysis of 4T1 using 67991-1-Ig (same clone as 67991-1-PBS)

    WB analysis of 4T1 using 67991-1-Ig (same clone as 67991-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67991-1-Ig (DDX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67991-1-Ig (same clone as 67991-1-PBS)

    WB analysis of HeLa using 67991-1-Ig (same clone as 67991-1-PBS)

    WB result of DDX1 antibody (67991-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX1 transfected HeLa cells. This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67991-1-Ig (same clone as 67991-1-PBS)

    IHC staining of mouse brain using 67991-1-Ig (same clone as 67991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse brain using 67991-1-Ig (same clone as 67991-1-PBS)

    IHC staining of mouse brain using 67991-1-Ig (same clone as 67991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse lung using 67991-1-Ig (same clone as 67991-1-PBS)

    IHC staining of mouse lung using 67991-1-Ig (same clone as 67991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse lung using 67991-1-Ig (same clone as 67991-1-PBS)

    IHC staining of mouse lung using 67991-1-Ig (same clone as 67991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse skin using 67991-1-Ig (same clone as 67991-1-PBS)

    IHC staining of mouse skin using 67991-1-Ig (same clone as 67991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse skin using 67991-1-Ig (same clone as 67991-1-PBS)

    IHC staining of mouse skin using 67991-1-Ig (same clone as 67991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of rat skin using 67991-1-Ig (same clone as 67991-1-PBS)

    IHC staining of rat skin using 67991-1-Ig (same clone as 67991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of rat skin using 67991-1-Ig (same clone as 67991-1-PBS)

    IHC staining of rat skin using 67991-1-Ig (same clone as 67991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67991-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DDX1 antibody included
  • Featured Product
  • KD/KO验证

DDX1 Monoclonal antibody, PBS Only

DDX1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67991-1-PBS

产品说明书

CloneNo. 1G10G4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

DEAD box protein retinoblastoma, DEAD box protein 1, DBP-RB, DBP RB, ATP-dependent RNA helicase DDX1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy DDX1 Ready-To-Use IHC Kit. DDX1 primary antibody included.

产品信息

67991-1-PBS targets DDX1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16774

Product name: Recombinant human DDX1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 391-740 aa of BC012132

Sequence: IPQVTSDGKRLQVIVCSATLHSFDVKKLSEKIMHFPTWVDLKGEDSVPDTVHHVVVPVNPKTDRLWERLGKSHIRTDDVHAKDNTRPGANSPEMWSEAIKILKGEYAVRAIKEHKMDQAIIFCRTKIDCDNLEQYFIQQGGGPDKKGHQFSCVCLHGDRKPHERKQNLERFKKGDVRFLICTDVAARGIDIHGVPYVINVTLPDEKQNYVHRIGRVGRAERMGLAISLVATEKEKVWYHVCSSRGKGCYNTRLKEDGGCTIWYNEMQLLSEIEEHLNCTISQVEPDIKVPVDEFDGKVTYGQKRAAGGGSYKGHVDILAPTVQELAALEKEAQTSFLHLGYLPNQLFRTF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
别名 DEAD box protein retinoblastoma, DEAD box protein 1, DBP-RB, DBP RB, ATP-dependent RNA helicase DDX1
计算分子量 740 aa, 82 kDa
观测分子量82 kDa
GenBank蛋白编号BC012132
基因名称 DDX1
Gene ID (NCBI) 1653
RRIDAB_2918740
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ92499
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX1 is a DEAD box protein, which is putative RNA helicases with a characteristic asp-glu-ala-asp (DEAD) box motif. DEAD box proteins involve in translation initiation, splicing, and ribosome and spliceosome assembly by altering RNA secondary structure. As a RNA helicase, DDX1 has a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome.