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验证数据展示

  • WB analysis using 67991-1-Ig

    WB analysis using 67991-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67991-1-Ig (DDX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of 4T1 using 67991-1-Ig

    WB analysis of 4T1 using 67991-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67991-1-Ig (DDX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67991-1-Ig

    WB analysis of HeLa using 67991-1-Ig

    WB result of DDX1 antibody (67991-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX1 transfected HeLa cells.

  • IHC staining of mouse brain using 67991-1-Ig

    IHC staining of mouse brain using 67991-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse brain using 67991-1-Ig

    IHC staining of mouse brain using 67991-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse lung using 67991-1-Ig

    IHC staining of mouse lung using 67991-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse lung using 67991-1-Ig

    IHC staining of mouse lung using 67991-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse skin using 67991-1-Ig

    IHC staining of mouse skin using 67991-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of mouse skin using 67991-1-Ig

    IHC staining of mouse skin using 67991-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of rat skin using 67991-1-Ig

    IHC staining of rat skin using 67991-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX1 antibody included
  • IHC staining of rat skin using 67991-1-Ig

    IHC staining of rat skin using 67991-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 67991-1-Ig (DDX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DDX1 antibody included
  • Featured Product
  • KD/KO验证

DDX1 Monoclonal antibody

DDX1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67991-1-Ig

产品说明书

CloneNo. 1G10G4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

DEAD box protein retinoblastoma, DEAD box protein 1, DBP-RB, DBP RB, ATP-dependent RNA helicase DDX1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, 4T1 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue, mouse lung tissue, mouse skin tissue, rat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DDX1 Ready-To-Use IHC Kit. DDX1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67991-1-Ig targets DDX1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16774

Product name: Recombinant human DDX1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 391-740 aa of BC012132

Sequence: IPQVTSDGKRLQVIVCSATLHSFDVKKLSEKIMHFPTWVDLKGEDSVPDTVHHVVVPVNPKTDRLWERLGKSHIRTDDVHAKDNTRPGANSPEMWSEAIKILKGEYAVRAIKEHKMDQAIIFCRTKIDCDNLEQYFIQQGGGPDKKGHQFSCVCLHGDRKPHERKQNLERFKKGDVRFLICTDVAARGIDIHGVPYVINVTLPDEKQNYVHRIGRVGRAERMGLAISLVATEKEKVWYHVCSSRGKGCYNTRLKEDGGCTIWYNEMQLLSEIEEHLNCTISQVEPDIKVPVDEFDGKVTYGQKRAAGGGSYKGHVDILAPTVQELAALEKEAQTSFLHLGYLPNQLFRTF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
别名 DEAD box protein retinoblastoma, DEAD box protein 1, DBP-RB, DBP RB, ATP-dependent RNA helicase DDX1
计算分子量 740 aa, 82 kDa
观测分子量82 kDa
GenBank蛋白编号BC012132
基因名称 DDX1
Gene ID (NCBI) 1653
RRIDAB_2918740
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ92499
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX1 is a DEAD box protein, which is putative RNA helicases with a characteristic asp-glu-ala-asp (DEAD) box motif. DEAD box proteins involve in translation initiation, splicing, and ribosome and spliceosome assembly by altering RNA secondary structure. As a RNA helicase, DDX1 has a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome.

实验方案

Product Specific Protocols
WB protocol for DDX1 antibody 67991-1-IgDownload protocol
IHC protocol for DDX1 antibody 67991-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols