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验证数据展示

  • WB analysis using 86667-2-RR (same clone as 86667-2-PBS)

    WB analysis using 86667-2-RR (same clone as 86667-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86667-2-RR (DDRGK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86667-2-PBS in a different storage buffer formulation.

  • WB analysis using 86667-2-RR (same clone as 86667-2-PBS)

    WB analysis using 86667-2-RR (same clone as 86667-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86667-2-RR (DDRGK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86667-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 86667-2-RR (same clone as 86667-2-PBS)

    IF Staining of HepG2 using 86667-2-RR (same clone as 86667-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDRGK1 antibody (86667-2-RR, Clone: 251582G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86667-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02579-1

    Cytometric bead array sample test of MP02579-1

    The mean DDRGK1 concentration was determined to be 109.2 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load, 36.0 ng/mL in PC-3 cell extract based on a 1.3 mg/mL extract load.

  • Cytometric bead array standard curve of MP02579-1

    Cytometric bead array standard curve of MP02579-1

    Cytometric bead array standard curve of MP02579-1, DDRGK1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86667-2-PBS. Detection antibody: 86667-1-PBS. Standard: Ag13742. Range: 0.469-60 ng/mL.

DDRGK1 Recombinant monoclonal antibody, PBS Only (Capture)

DDRGK1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86667-2-PBS

产品说明书

CloneNo. 251582G2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

C20orf116, Dashurin, DDRGK domain containing 1, DDRGK domain-containing protein 1, UFBP1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DDRGK1 Ready-To-Use IHC Kit. DDRGK1 primary antibody included.

产品信息

86667-2-PBS targets DDRGK1 as part of a matched antibody pair:

MP02579-1: 86667-2-PBS capture and 86667-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13742

Product name: Recombinant human DDRGK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-314 aa of BC000643

Sequence: MVAPVWYLVAAALLVGFILFLTRSRGRAASAGQEPLHNEELAGAGRVAQPGPLEPEEPRAGGRPRRRRDLGSRLQAQRRAQRVAWAEADENEEEAVILAQEEEGVEKPAETHLSGKIGAKKLRKLEEKQARKAQREAEEAEREERKRLESQREAEWKKEEERLRLEEEQKEEEERKAREEQAQREHEEYLKLKEAFVVEEEGVGETMTEEQSQSFLTEFINYIKQSKVVLLEDLASQVGLRTQDTINRIQDLLAEGTITGVIDDRGKFIYITPEELAAVANFIRQRGRVSIAELAQASNSLIAWGRESPAQAPA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DDRGK domain containing 1
别名 C20orf116, Dashurin, DDRGK domain containing 1, DDRGK domain-containing protein 1, UFBP1
计算分子量 314 aa, 36 kDa
观测分子量 36-46 kDa
GenBank蛋白编号BC000643
基因名称 DDRGK1
Gene ID (NCBI) 65992
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96HY6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDRGK1, also name as C20orf116, is a member of the DDRGK domain-containing protein family with unknown function. Depletion of DDRGK1 inhibits cell proliferation and invasion. Expression of NF-κB target genes showed the most significant decrease after depleting of DDRGK1, suggesting that DDRGK1 may play an important role in the NF-κB signaling pathway. DDRGK1 interacts with IκBα and regulates its stability, thereby regulates the NF-κB transcriptional activity. DDRGK1 acts as an important regulator of the NF-κB pathway. (PMID: 23675531)

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