验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells, HepG2 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
67126-1-Ig targets DDR2 in WB, IF, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag28340 Product name: Recombinant human DDR2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 291-398 aa of NM_001014796 Sequence: MFAKGVKIFKEVQCYFRSEASEWEPNAISFPLVLDDVNPSARFVTVPLHHRMASAIKCQYHFADTWMMFSEITFQSDAAMYNNSEALPTSPMAPTTYDPMLKVDDSNT 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | discoidin domain receptor tyrosine kinase 2 |
| 别名 | DDR2, Discoidin domain receptor 2, MIG20a, NTRKR3, TKT, TYRO10, Tyrosine protein kinase TYRO10 |
| 计算分子量 | 97 kDa |
| 观测分子量 | 100-110 kDa |
| GenBank蛋白编号 | NM_001014796 |
| 基因名称 | DDR2 |
| Gene ID (NCBI) | 4921 |
| RRID | AB_2882426 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q16832 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DDR2 (Discoidin domain receptor 2) belongs to the receptor tyrosine kinase (RTK) family and is activated by collagen-binding.DDR2 is implicated in several physiological and pathological processes, including wound healing, angiogenesis, ovulation, spermatogenesis, extracellular matrix (ECM) remodeling and fibrosis, and tumor progression (PMID: 25805889). Moreover, DDR2 is prominently present in the fibroblasts, smooth muscle cells, myofibroblasts, and chondrocytes (PMID: 37834343).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DDR2 antibody 67126-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Mol Med The role and mechanism of transforming growth factor beta 3 in human myocardial infarction-induced myocardial fibrosis. | ||
Int J Biol Macromol Human type III collagen-derived biomaterial with high antitumor activity inhibits breast cancer cell autophagy, proliferation, and migration through DDR1 |
