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验证数据展示

  • WB analysis using 25377-1-AP (same clone as 25377-1-PBS)

    WB analysis using 25377-1-AP (same clone as 25377-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25377-1-AP (DDI2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25377-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 25377-1-AP (same clone as 25377-1-PBS)

    IF Staining of HepG2 using 25377-1-AP (same clone as 25377-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDI2 antibody (25377-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25377-1-PBS in a different storage buffer formulation.

DDI2 Polyclonal antibody, PBS Only

DDI2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 25377-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

Protein DDI1 homolog 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25377-1-PBS targets DDI2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21953

Product name: Recombinant human DDI2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 22-62 aa of BC006011

Sequence: DADFELHNFRALCELESGIPAAESQIVYAERPLTDNHRSLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DDI1, DNA-damage inducible 1, homolog 2 (S. cerevisiae)
别名 Protein DDI1 homolog 2
计算分子量 399 aa, 45 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC006011
基因名称 DDI2
Gene ID (NCBI) 84301
RRIDAB_3669472
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5TDH0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDI2 contains 399 amino acids and contains an N-terminal ubiquitin-like (UBL) domain and a highly conserved retroviral protease-like (RVP) domain. DDI2 is an aspartic endoprotease that cleaves ubiquitylated target proteins to assist in their processing by the ubiquitin-proteasome system (UPS), especially when the UPS is inhibited(PMID: 32521225).

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