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验证数据展示

  • WB analysis using 25377-1-AP

    WB analysis using 25377-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25377-1-AP (DDI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

DDI2 Polyclonal antibody

DDI2 Polyclonal Antibody for WB, ELISA
Cat No. 25377-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Protein DDI1 homolog 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HUVEC cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25377-1-AP targets DDI2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 DDI2 fusion protein Ag21953 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DDI1, DNA-damage inducible 1, homolog 2 (S. cerevisiae)
别名 Protein DDI1 homolog 2
计算分子量 399 aa, 45 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC006011
基因名称 DDI2
Gene ID (NCBI) 84301
RRIDAB_3669472
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5TDH0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDI2 contains 399 amino acids and contains an N-terminal ubiquitin-like (UBL) domain and a highly conserved retroviral protease-like (RVP) domain. DDI2 is an aspartic endoprotease that cleaves ubiquitylated target proteins to assist in their processing by the ubiquitin-proteasome system (UPS), especially when the UPS is inhibited(PMID: 32521225).

实验方案

Product Specific Protocols
WB protocol for DDI2 antibody 25377-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols