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验证数据展示

  • WB analysis using 14966-1-AP (same clone as 14966-1-PBS)

    WB analysis using 14966-1-AP (same clone as 14966-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 14966-1-AP (same clone as 14966-1-PBS)

    WB analysis of MCF-7 using 14966-1-AP (same clone as 14966-1-PBS)

    WB result of DDAH2 antibody (14966-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDAH2 transfected MCF-7 cells. This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

  • IP experiment of COLO 320 using 14966-1-AP (same clone as 14966-1-PBS)

    IP experiment of COLO 320 using 14966-1-AP (same clone as 14966-1-PBS)

    IP result of anti-DDAH2 (IP:14966-1-AP, 3ug; Detection:14966-1-AP 1:500) with COLO 320 cells lysate 2000ug. This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 14966-1-AP (same clone as 14966-1-PBS)

    IHC staining of human colon cancer using 14966-1-AP (same clone as 14966-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 14966-1-AP (same clone as 14966-1-PBS)

    IHC staining of human colon cancer using 14966-1-AP (same clone as 14966-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DDAH2 Polyclonal antibody, PBS Only

DDAH2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 14966-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

DDAH, DDAH 2, DDAH-2, DDAHII, Dimethylargininase 2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14966-1-PBS targets DDAH2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6831

Product name: Recombinant human DDAH2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-285 aa of BC001435

Sequence: MGTPGEGLGRCSHALIRGVPESLASGEGAGAGLPALDLAKAQREHGVLGGKLRQRLGLQLLELPPEESLPLGPLLGDTAVIQGDTALITRPWSPARRPEVDGVRKALQDLGLRIVEIGDENATLDGTDVLFTGREFFVGLSKWTNHRGAEIVADTFRDFAVSTVPVSGPSHLRGLCGMGGPRTVVAGSSDAAQKAVRAMAVLTDHPYASLTLPDDAAADCLFLRPGLPGVPPFLLHRGGGDLPNSQEALQKLSDVTLVPVSCSELEKAGAGLSSLCLVLSTRPHS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dimethylarginine dimethylaminohydrolase 2
别名 DDAH, DDAH 2, DDAH-2, DDAHII, Dimethylargininase 2
计算分子量 29 kDa
观测分子量 30 kDa
GenBank蛋白编号BC001435
基因名称 DDAH2
Gene ID (NCBI) 23564
RRIDAB_2276973
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95865
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The DDAH family of proteins is formed by two isoforms, DDAH-1 and DDAH-2, both of which utilize ADMA as their substrate and increase NOS activity. DDAH-2,also named as DDAH, G6A, NG30, is the most prevalent isoform in blood vessels and endothelium and is important for embryonic development. It has also been linked to other diseases, such as hypertension, cerebral hemorrhage, cardiac disease, and diabetes. In cultured human endothelial cells,DDAH-2 had been found only in the cytosol, but in the nuclei of rat vascular smooth muscle cells.(PMID:21898353).

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