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验证数据展示

  • WB analysis using 11325-1-AP (same clone as 11325-1-PBS)

    WB analysis using 11325-1-AP (same clone as 11325-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11325-1-AP (DCTN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11325-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 11325-1-AP (same clone as 11325-1-PBS)

    IHC staining of mouse brain using 11325-1-AP (same clone as 11325-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11325-1-AP (DCTN3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11325-1-PBS in a different storage buffer formulation.

DCTN3 Polyclonal antibody, PBS Only

DCTN3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11325-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

DCTN 22, DCTN 3, DCTN22, dynactin 3 (p22), p22

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11325-1-PBS targets DCTN3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1865

Product name: Recombinant human DCTN3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-176 aa of BC000319

Sequence: MAGLTDLQRLQARVEELERWVYGPGGARGSRKVADGLVKVQVALGNISSKRERVKILYKKIEDLIKYLDPEYIDRIAIPDASKLQFILAEEQFILSQVALLEQVNALVPMLDSAHIKAVPEHAARLQRLAQIHIQQQAPWGVGVRDEAGSLVEDVGFAQFLSVLHFGPTGPVCGNH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dynactin 3 (p22)
别名 DCTN 22, DCTN 3, DCTN22, dynactin 3 (p22), p22
计算分子量 186 aa, 21 kDa
观测分子量22 kDa
GenBank蛋白编号BC000319
基因名称 DCTN3
Gene ID (NCBI) 11258
RRIDAB_3741816
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75935
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DCTN3 (Dynactin Subunit 3, also known as p22) is a core component of the dynactin complex, essential for cytoplasmic dynein-mediated intracellular transport. This 22-kDa polypeptide binds directly to microtubules and stabilizes the dynactin structure, facilitating cargo movement along microtubule tracks. DCTN3 plays critical roles in mitotic spindle organization, vesicle trafficking, and neuronal development (PMID: 24352458).

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