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验证数据展示

  • WB analysis of A375 using 68114-1-Ig

    WB analysis of A375 using 68114-1-Ig

    A375 cells were subjected to SDS PAGE followed by western blot with 68114-1-Ig (DCT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 68114-1-Ig

    IF Staining of A549 using 68114-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using DCT antibody (68114-1-Ig, Clone: 1B3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

DCT Monoclonal antibody

DCT Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68114-1-Ig

产品说明书

CloneNo. 1B3D4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, pig

应用

WB, IF/ICC, ELISA

别名

1B3D4, DT, EC:5.3.3.12, L-dopachrome Delta-isomerase, L-dopachrome tautomerase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA375 cells, HL-60 cells, pig skin tissue, mouse skin tissue, mouse testis tissue
Positive IF/ICC detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68114-1-Ig targets DCT in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, pig
免疫原

CatNo: Ag26165

Product name: Recombinant human DCT protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC028311

Sequence: MSPLWWGFLLSCLGCKILPGAQGQFPRVCMTVDSLVNKECCPRLGAESANVCGSQQGRGQCTEVRADTRPWSGPYILRNQDDRELWPRKFFHRTCKCTGNFAGYNCGDCKFGWTGPNCERKKPPVIRQNIHSLSPQEREQFLGALDLAKKRVHPDYVITTQHWLGLLGPNGTQPQFANCSVYDFFVWLHYYSVRDTLLGPGRPYRAIDFSHQGPAFVTWHRYHLLCLERDLQRLIGNESFALPYWNFATGRNECDVCTDQLFGAARPDDPTLISRNSRFSSWETVCDSLDDYNHLVTLCNGTYEGLLRRNQMGRNSMKLPTLKDIRDCLSLQKFDNPPFFQNSTFSFRNA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
别名 1B3D4, DT, EC:5.3.3.12, L-dopachrome Delta-isomerase, L-dopachrome tautomerase
计算分子量 519 aa, 59 kDa
观测分子量55-63 kDa
GenBank蛋白编号BC028311
基因名称 DCT
Gene ID (NCBI) 1638
RRIDAB_2923643
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP40126
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).

实验方案

Product Specific Protocols
IF protocol for DCT antibody 68114-1-IgDownload protocol
WB protocol for DCT antibody 68114-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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