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DCPS Monoclonal antibody

DCPS Monoclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B2E4

Cat No : 67311-1-Ig

Print datasheet

Synonyms

DCPS, DCS 1, DCS1, decapping enzyme, scavenger, HINT 5, HINT5, HSL1, HSPC015

验证数据展示

  • WB analysis using 67311-1-Ig

    WB analysis using 67311-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67311-1-Ig (DCPS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67311-1-Ig

    WB analysis of HeLa using 67311-1-Ig

    WB result of DCPS antibody (67311-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCPS transfected HeLa cells.

  • IHC staining of human breast cancer using 67311-1-Ig

    IHC staining of human breast cancer using 67311-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67311-1-Ig (DCPS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DCPS antibody included
  • IHC staining of human breast cancer using 67311-1-Ig

    IHC staining of human breast cancer using 67311-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67311-1-Ig (DCPS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DCPS antibody included
  • IF Staining of human liver cancer using 67311-1-Ig

    IF Staining of human liver cancer using 67311-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using DCPS antibody (67311-1-Ig, Clone: 1B2E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, rat spleen tissue, mouse brain tissue, HepG2 cells, K-562 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue
Planning an IHC experiment? We recommend our IHCeasy DCPS Ready-To-Use IHC Kit. DCPS primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67311-1-Ig targets DCPS in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF-P, IHC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen DCPS fusion protein Ag8872 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name decapping enzyme, scavenger
Synonyms DCPS, DCS 1, DCS1, decapping enzyme, scavenger, HINT 5, HINT5, HSL1, HSPC015
Calculated Molecular Weight 337 aa, 39 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC014532
Gene Symbol DCPS
Gene ID (NCBI) 28960
RRIDAB_2882571
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96C86
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DcpS, which is a member of the histidine triad (HIT) superfamily of pyrophosphatases, is the first HIT protein with a defined biological function and identifies the HIT motif as a new mRNA decapping domain. DcpS can encode a 38-40 kDa protein, and has the potential to form a homodimer of 80 kDa(PMID: 12198172).

实验方案

Product Specific Protocols
WB protocol for DCPS antibody 67311-1-IgDownload protocol
IHC protocol for DCPS antibody 67311-1-IgDownload protocol
IF protocol for DCPS antibody 67311-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols