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验证数据展示

  • WB analysis using 28465-1-AP (same clone as 28465-1-PBS)

    WB analysis using 28465-1-AP (same clone as 28465-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28465-1-AP (DCPS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28465-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 28465-1-AP (same clone as 28465-1-PBS)

    IF Staining of HeLa using 28465-1-AP (same clone as 28465-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DCPS antibody (28465-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 28465-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 28465-1-AP (same clone as 28465-1-PBS)

    IHC staining of mouse testis using 28465-1-AP (same clone as 28465-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28465-1-AP (DCPS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28465-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DCPS antibody included
  • IHC staining of human kidney using 28465-1-AP (same clone as 28465-1-PBS)

    IHC staining of human kidney using 28465-1-AP (same clone as 28465-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28465-1-AP (DCPS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28465-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DCPS antibody included

DCPS Polyclonal antibody, PBS Only

DCPS Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28465-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DCS 1, DCS1, DCS-1, decapping enzyme, scavenger, Decapping scavenger enzyme

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DCPS Ready-To-Use IHC Kit. DCPS primary antibody included.

产品信息

28465-1-PBS targets DCPS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag29351

Product name: Recombinant human DCPS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 7-160 aa of BC014532

Sequence: QLGKRKRELDVEEAHAASTEEKEAGVGNGTCAPVRLPFSGFRLQKVLRESARDKIIFLHGKVNEASEDGDGEDAVVILEKTPFQVEQVAQLLTGSPELQLQFSNDIYSTYHLFPPRQLNDVKTTVVYPATEKHLQKYLRQDLRLIRETGDDYRN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 decapping enzyme, scavenger
别名 DCS 1, DCS1, DCS-1, decapping enzyme, scavenger, Decapping scavenger enzyme
计算分子量 337 aa, 39 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC014532
基因名称 DCPS
Gene ID (NCBI) 28960
RRIDAB_3086054
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96C86
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DcpS, which is a member of the histidine triad (HIT) superfamily of pyrophosphatases, is the first HIT protein with a defined biological function and identifies the HIT motif as a new mRNA decapping domain. DcpS can encode a 38-40 kDa protein, and has the potential to form a homodimer of 80 kDa(PMID: 12198172).

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