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Species Reactivity
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验证数据展示

  • WB analysis using 28465-1-AP

    WB analysis using 28465-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28465-1-AP (DCPS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 28465-1-AP

    IF Staining of HeLa using 28465-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DCPS antibody (28465-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

DCPS Polyclonal antibody

DCPS Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28465-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

EC:3.6.1.59, Decapping scavenger enzyme, decapping enzyme, scavenger, DCS-1, DCS1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, mouse testis tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DCPS Ready-To-Use IHC Kit. DCPS primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28465-1-AP targets DCPS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 DCPS fusion protein Ag29351 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 decapping enzyme, scavenger
别名 EC:3.6.1.59, Decapping scavenger enzyme, decapping enzyme, scavenger, DCS-1, DCS1
计算分子量 337 aa, 39 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC014532
基因名称 DCPS
Gene ID (NCBI) 28960
RRIDAB_3086054
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96C86
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DcpS, which is a member of the histidine triad (HIT) superfamily of pyrophosphatases, is the first HIT protein with a defined biological function and identifies the HIT motif as a new mRNA decapping domain. DcpS can encode a 38-40 kDa protein, and has the potential to form a homodimer of 80 kDa(PMID: 12198172).

实验方案

Product Specific Protocols
WB protocol for DCPS antibody 28465-1-APDownload protocol
IF protocol for DCPS antibody 28465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols