验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, mouse testis tissue |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28465-1-AP targets DCPS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | DCPS fusion protein Ag29351 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | decapping enzyme, scavenger |
别名 | EC:3.6.1.59, Decapping scavenger enzyme, decapping enzyme, scavenger, DCS-1, DCS1 |
计算分子量 | 337 aa, 39 kDa |
观测分子量 | 35-40 kDa |
GenBank蛋白编号 | BC014532 |
基因名称 | DCPS |
Gene ID (NCBI) | 28960 |
RRID | AB_3086054 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q96C86 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DcpS, which is a member of the histidine triad (HIT) superfamily of pyrophosphatases, is the first HIT protein with a defined biological function and identifies the HIT motif as a new mRNA decapping domain. DcpS can encode a 38-40 kDa protein, and has the potential to form a homodimer of 80 kDa(PMID: 12198172).
实验方案
Product Specific Protocols | |
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WB protocol for DCPS antibody 28465-1-AP | Download protocol |
IF protocol for DCPS antibody 28465-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |