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验证数据展示

  • IF Staining of mouse brain using CL488-68234

    IF Staining of mouse brain using CL488-68234

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 DCLK1 antibody (CL488-68234, Clone: 3C12C1 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated DCLK1 Monoclonal antibody

DCLK1 Monoclonal Antibody for IF-P
Cat No. CL488-68234

产品说明书

CloneNo. 3C12C1

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, rabbit, pig

应用

IF-P

别名

DCAMKL1, DCDC3A, DCLK, DCLK1, doublecortin like kinase 1, KIAA0369

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 68234-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68234 targets DCLK1 in IF-P applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

经测试应用 IF-P Application Description
经测试反应性 Human, mouse, rat, rabbit, pig
免疫原

CatNo: Ag17110

Product name: Recombinant human DCLK1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 623-729 aa of BC152456

Sequence: LITMMLLVDVDQRFSAVQVLEHPWVNDDGLPENEHQLSVAGKIKKHFNTGPKPNSTAAGVSVIALDHGFTIKRSGSLDYYQQPGMYWIRPPLLIRRGRFSDEDATRM

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 doublecortin-like kinase 1
别名 DCAMKL1, DCDC3A, DCLK, DCLK1, doublecortin like kinase 1, KIAA0369
计算分子量 729 aa, 81 kDa
观测分子量46 kDa, 82 kDa
GenBank蛋白编号BC152456
基因名称 DCLK1
Gene ID (NCBI) 9201
RRIDAB_3084450
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDO15075
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCLK1 (Serine/threonine-protein kinase DCLK1) is also named as DCAMKL1, DCDC3A, KIAA0369 and belongs to the CAMK Ser/Thr protein kinase family. It is a microtubule-associated kinase that can undergo autophosphorylation and it also has microtubule-polymerizing activity that is independent of its protein kinase activity (PMID: 11124993). It plays a unique role in mitotic spindle integrity during early neurogenesis in radial glial cell proliferation and their radial process stability. DCLK1 is a unique marker for distinguishing tumor stem cells from intestinal normal stem cells (PMID: 23202126). This protein has 4 isoforms produced by alternative splicing with the molecular weight of 82 kDa, 81 kDa, 47 kDa and 48 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DCLK1 antibody CL488-68234Download protocol
Standard Protocols
Click here to view our Standard Protocols