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验证数据展示

  • WB analysis using 11535-1-AP (same clone as 11535-1-PBS)

    WB analysis using 11535-1-AP (same clone as 11535-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

  • WB analysis of MOVAS using 11535-1-AP (same clone as 11535-1-PBS)

    WB analysis of MOVAS using 11535-1-AP (same clone as 11535-1-PBS)

    WB result of DCI antibody (11535-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and DCI knockout MOVAS cells. This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 11535-1-AP (same clone as 11535-1-PBS)

    IHC staining of mouse liver using 11535-1-AP (same clone as 11535-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11535-1-AP (DCI antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 11535-1-AP (same clone as 11535-1-PBS)

    IHC staining of mouse liver using 11535-1-AP (same clone as 11535-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11535-1-AP (DCI antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 11535-1-AP (same clone as 11535-1-PBS)

    IF Staining of HEK-293 using 11535-1-AP (same clone as 11535-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DCI antibody (11535-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11535-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DCI Polyclonal antibody, PBS Only

DCI Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11535-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

3,2-trans-enoyl-CoA isomerase, D3,D2-enoyl-CoA isomerase, Delta(3),Delta(2)-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, EC:5.3.3.8

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11535-1-PBS targets DCI in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2094

Product name: Recombinant human DCI protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-285 aa of BC020228

Sequence: MALVASVRVPARVLLRAGARLPGAALGRTERAAGGGDGARRFGSQRVLVEPDAGAGVAVMKFKNPPVNSLSLEFLTELVISLEKLENDKSFRGVILTSDRPGVFSAGLDLTEMCGRSPAHYAGYWKAVQELWLRLYQSNLVLVSAINGACPAGGCLVALTCDYRILADNPRLKDTLENTIGHRAAERALQLGLLFPPAEALQVGIVDQVVPEEQVQSTALSAIAQWMAIPDHARQLTKAMMRKATASRLVTQRDADVQNFVSFISKDSIQKSLQMYLERLKEEKG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase)
别名 3,2-trans-enoyl-CoA isomerase, D3,D2-enoyl-CoA isomerase, Delta(3),Delta(2)-enoyl-CoA isomerase, Dodecenoyl-CoA isomerase, EC:5.3.3.8
计算分子量 302 aa, 33 kDa
观测分子量 31 kDa
GenBank蛋白编号BC020228
基因名称 DCI
Gene ID (NCBI) 1632
RRIDAB_2089906
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42126
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ECI1(enoyl-CoA delta isomerase 1, mitochondrial) is also named as DCI(dodecenoyl-CoA isomerase) and belongs to the enoyl-CoA hydratase/isomerase family. It is able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. The full length protein has a transit peptide and 2 isoforms produced by alternative splicing and can exsit as a homotramer(PMID:15351645).

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