• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

DCI Polyclonal antibody

DCI Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11535-1-AP

Print datasheet

Synonyms

D3,D2 enoyl CoA isomerase, DCI, Dodecenoyl CoA isomerase, ECI1

验证数据展示

  • WB analysis using 11535-1-AP

    WB analysis using 11535-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11535-1-AP (DCI antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 11535-1-AP

    IHC staining of human prostate cancer using 11535-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11535-1-AP (DCI antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of BxPC-3 using 11535-1-AP

    IF Staining of BxPC-3 using 11535-1-AP

    Immunofluorescent analysis of BxPC-3 cells using 11535-1-AP (DCI antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, human lung tissue, human brain tissue, human kidney tissue, BxPC-3 cells, COLO 320 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inBxPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11535-1-AP targets DCI in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen DCI fusion protein Ag2094 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase)
Synonyms D3,D2 enoyl CoA isomerase, DCI, Dodecenoyl CoA isomerase, ECI1
Calculated Molecular Weight 302 aa, 33 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC020228
Gene Symbol DCI
Gene ID (NCBI) 1632
RRIDAB_2089906
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42126
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ECI1(enoyl-CoA delta isomerase 1, mitochondrial) is also named as DCI(dodecenoyl-CoA isomerase) and belongs to the enoyl-CoA hydratase/isomerase family. It is able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. The full length protein has a transit peptide and 2 isoforms produced by alternative splicing and can exsit as a homotramer(PMID:15351645).

实验方案

Product Specific Protocols
WB protocol for DCI antibody 11535-1-APDownload protocol
IHC protocol for DCI antibody 11535-1-APDownload protocol
IF protocol for DCI antibody 11535-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols