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验证数据展示

  • WB analysis using 28144-1-AP (same clone as 28144-1-PBS)

    WB analysis using 28144-1-AP (same clone as 28144-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 28144-1-AP (DCHS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28144-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 28144-1-AP (same clone as 28144-1-PBS)

    IF Staining of mouse brain using 28144-1-AP (same clone as 28144-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using DCHS1 antibody (28144-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28144-1-PBS in a different storage buffer formulation.

DCHS1 Polyclonal antibody, PBS Only

DCHS1 Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 28144-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, Indirect ELISA

别名

Cadherin 19, Cadherin 25, CDH19, CDH25, dachsous 1 (Drosophila)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28144-1-PBS targets DCHS1 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag27226

Product name: Recombinant human DCHS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1517-1718 aa of NM_003737

Sequence: MPANASRRRAARVSARVFVTDENDNAPVFASPSRVRLPEDQPPGPAALHVVARDPDLGEAARVSYRLASGGDGHFRLHSSTGALSVVRPLDREQRAEHVLTVVASDHGSPPRSATQVLTVSVADVNDEAPTFQQQEYSVLLRENNPPGTSLLTLRATDPDVGANGQVTYGGVSSESFSLDPDTGVLTTLRALDREEQEEIN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dachsous 1 (Drosophila)
别名 Cadherin 19, Cadherin 25, CDH19, CDH25, dachsous 1 (Drosophila)
计算分子量 346 kDa
观测分子量~350 kDa
GenBank蛋白编号NM_003737
基因名称 DCHS1
Gene ID (NCBI) 8642
RRIDAB_3669648
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96JQ0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DCHS1 is one of two known pathogenic genes associated with mitral valve prolapse (MVP) , a common cardiac valve disease (PMID: 33225636). Dchs1 and Fat4 function as a ligand-receptor pair during murine development and these genes are required in multiple organs (PMID: 21303848).

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