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验证数据展示

  • WB analysis using 85000-4-RR

    WB analysis using 85000-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85000-4-RR (DCBLD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of U2OS using 85000-4-RR

    IP experiment of U2OS using 85000-4-RR

    IP result of anti-DCBLD2 (IP:85000-4-RR, 4ug; Detection:85000-4-RR 1:2000) with U2OS cells lysate 1120 ug.

  • IHC staining of human colon cancer using 85000-4-RR

    IHC staining of human colon cancer using 85000-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 85000-4-RR

    IHC staining of human colon cancer using 85000-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85000-4-RR

    Affinity and Kinetic Characterization of 85000-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85000-4-RR against Human DCBLD2 were performed. The affinity constant is 0.170 nM.

DCBLD2 Recombinant antibody

DCBLD2 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, ELISA
Cat No. 85000-4-RR

产品说明书

CloneNo. 242604E8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

242604E8, CLCP1, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, Discoidin, CUB and LCCL domain-containing protein 2, Endothelial and smooth muscle cell-derived neuropilin-like protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, U2OS cells
Positive IP detected inU2OS cells
Positive IHC detected inhuman colon cancer tissue, human rectal cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85000-4-RR targets DCBLD2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human
免疫原 DCBLD2 fusion protein Ag32823 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 discoidin, CUB and LCCL domain containing 2
别名 242604E8, CLCP1, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, Discoidin, CUB and LCCL domain-containing protein 2, Endothelial and smooth muscle cell-derived neuropilin-like protein
计算分子量 775 aa, 89 kDa
观测分子量120 kDa
GenBank蛋白编号BC029658
基因名称 DCBLD2
Gene ID (NCBI) 131566
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96PD2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DCBLD2, also known as ESDN or CLCP1, was first identified from human coronary arterial cells by a signal sequence trap method. DCBLD2 regulates proliferation, migration, and invasion in various tumors, including glioma, hypopharyngeal squamous cell carcinoma, and myxofibrosarcoma. The human DCBLD2 protein has a predicted molecular mass of ∼80 kDa based on its amino acid sequence, although it regularly displays an effective molecular mass of ∼130 kDa via SDS-PAGE (PMID: 30902898).

实验方案

Product Specific Protocols
WB protocol for DCBLD2 antibody 85000-4-RRDownload protocol
IHC protocol for DCBLD2 antibody 85000-4-RRDownload protocol
IP protocol for DCBLD2 antibody 85000-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols