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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85000-4-RR (same clone as 85000-4-PBS)

    WB analysis using 85000-4-RR (same clone as 85000-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85000-4-RR (DCBLD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

  • IP experiment of U2OS using 85000-4-RR (same clone as 85000-4-PBS)

    IP experiment of U2OS using 85000-4-RR (same clone as 85000-4-PBS)

    IP result of anti-DCBLD2 (IP:85000-4-RR, 4ug; Detection:85000-4-RR 1:2000) with U2OS cells lysate 1120 ug. This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 85000-4-RR (same clone as 85000-4-PBS)

    IHC staining of human colon cancer using 85000-4-RR (same clone as 85000-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 85000-4-RR (same clone as 85000-4-PBS)

    IHC staining of human colon cancer using 85000-4-RR (same clone as 85000-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85000-4-RR

    Affinity and Kinetic Characterization of 85000-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85000-4-RR against Human DCBLD2 were performed. The affinity constant is 0.170 nM.

    {{ptg:ProductFeaturesInfo}}

DCBLD2 Recombinant monoclonal antibody, PBS Only

DCBLD2 Uni-rAb® Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 85000-4-PBS

产品说明书

CloneNo. 242604E8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

242604E8, CLCP1, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, Discoidin, CUB and LCCL domain-containing protein 2, Endothelial and smooth muscle cell-derived neuropilin-like protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85000-4-PBS targets DCBLD2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag32823

Product name: Recombinant human DCBLD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 582-743 aa of BC029658

Sequence: LQADSAEYAQPLVGGIVGTLHQRSTFKPEEGKEAGYADLDPYNSPGQEVYHAYAEPLPITGPEYATPIIMDMSGHPTTSVGQPSTSTFKATGNQPPPLVGTYNTLLSRTDSCSSAQAQYDTPKAGKPGLPAPDELVYQVPQSTQEVSGAGRDGECDVFKEIL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 discoidin, CUB and LCCL domain containing 2
别名 242604E8, CLCP1, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, Discoidin, CUB and LCCL domain-containing protein 2, Endothelial and smooth muscle cell-derived neuropilin-like protein
计算分子量 775 aa, 89 kDa
观测分子量120 kDa
GenBank蛋白编号BC029658
基因名称 DCBLD2
Gene ID (NCBI) 131566
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ96PD2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DCBLD2, also known as ESDN or CLCP1, was first identified from human coronary arterial cells by a signal sequence trap method. DCBLD2 regulates proliferation, migration, and invasion in various tumors, including glioma, hypopharyngeal squamous cell carcinoma, and myxofibrosarcoma. The human DCBLD2 protein has a predicted molecular mass of ∼80 kDa based on its amino acid sequence, although it regularly displays an effective molecular mass of ∼130 kDa via SDS-PAGE (PMID: 30902898).