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Host
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Species Reactivity
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验证数据展示

  • WB analysis using 85000-4-RR (same clone as 85000-4-PBS)

    WB analysis using 85000-4-RR (same clone as 85000-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85000-4-RR (DCBLD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

  • IP experiment of U2OS using 85000-4-RR (same clone as 85000-4-PBS)

    IP experiment of U2OS using 85000-4-RR (same clone as 85000-4-PBS)

    IP result of anti-DCBLD2 (IP:85000-4-RR, 4ug; Detection:85000-4-RR 1:2000) with U2OS cells lysate 1120 ug. This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 85000-4-RR (same clone as 85000-4-PBS)

    IHC staining of human colon cancer using 85000-4-RR (same clone as 85000-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 85000-4-RR (same clone as 85000-4-PBS)

    IHC staining of human colon cancer using 85000-4-RR (same clone as 85000-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85000-4-RR (DCBLD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85000-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85000-4-RR

    Affinity and Kinetic Characterization of 85000-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85000-4-RR against Human DCBLD2 were performed. The affinity constant is 0.170 nM.

DCBLD2 Recombinant antibody, PBS Only

DCBLD2 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 85000-4-PBS

产品说明书

CloneNo. 242604E8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

242604E8, CLCP1, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, Discoidin, CUB and LCCL domain-containing protein 2, Endothelial and smooth muscle cell-derived neuropilin-like protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85000-4-PBS targets DCBLD2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 DCBLD2 fusion protein Ag32823 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 discoidin, CUB and LCCL domain containing 2
别名 242604E8, CLCP1, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, Discoidin, CUB and LCCL domain-containing protein 2, Endothelial and smooth muscle cell-derived neuropilin-like protein
计算分子量 775 aa, 89 kDa
观测分子量120 kDa
GenBank蛋白编号BC029658
基因名称 DCBLD2
Gene ID (NCBI) 131566
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ96PD2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DCBLD2, also known as ESDN or CLCP1, was first identified from human coronary arterial cells by a signal sequence trap method. DCBLD2 regulates proliferation, migration, and invasion in various tumors, including glioma, hypopharyngeal squamous cell carcinoma, and myxofibrosarcoma. The human DCBLD2 protein has a predicted molecular mass of ∼80 kDa based on its amino acid sequence, although it regularly displays an effective molecular mass of ∼130 kDa via SDS-PAGE (PMID: 30902898).