验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12662-1-AP targets DBP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, chicken |
| 免疫原 |
CatNo: Ag3343 Product name: Recombinant human DBP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-325 aa of BC011965 Sequence: MARPVSDRTPAPLLLGGPAGTPPGGGALLGLRSLLQGTSKPKEPASCLLKEKERKAALPAATTPGPGLETAGPADAPAGAVVGGGSPRGRPGPVPAPGLLAPLLWERTLPFGDVEYVDLDAFLLEHGLPPSPPPPGGPSPEPSPARTPAPSPGPGSCGSASPRSSPGHAPARAALGTASGHRAGLTSRDTPSPVDPDTVEVLMTFEPDPADLALSSIPGHETFDPRRHRFSEEELKPQPIMKKARKIQVPEEQKDEKYWSRRYKNNEAAKRSRDARRLKENQISVRAAFLEKENALLRQEVVAVRQELSHYRAVLSRYQAQHGAL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | D site of albumin promoter (albumin D-box) binding protein |
| 别名 | Albumin D box binding protein, D site binding protein, DABP, DBP, TaxREB302 |
| 计算分子量 | 325 aa, 34 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC011965 |
| 基因名称 | DBP |
| Gene ID (NCBI) | 1628 |
| RRID | AB_2877870 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q10586 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for DBP antibody 12662-1-AP | Download protocol |
| WB protocol for DBP antibody 12662-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Time-restricted feeding prevents deleterious metabolic effects of circadian disruption through epigenetic control of β cell function. | ||
Theranostics PPAR-γ integrates obesity and adipocyte clock through epigenetic regulation of Bmal1. | ||
FASEB J Rhythmic expression of miR-27b-3p targets the clock gene Bmal1 at the posttranscriptional level in the mouse liver. | ||
Am J Physiol Gastrointest Liver Physiol Parenteral nutrition results in peripheral ileal to hepatic circadian discordance in mice | ||
Commun Biol ISX-9 potentiates CaMKIIδ-mediated BMAL1 activation to enhance circadian amplitude. | ||
Sci Rep Gene expression profiles during tissue remodeling following bladder outlet obstruction.
|
