验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse testis tissue |
For other applications, we recommend the unconjugated version of this antibody, 86344-3-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-86344-3 targets DAZL in IF-P applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag37074 Product name: Recombinant human DAZL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 200-295 aa of BC027595 Sequence: QRSYVVPPAYSAVNYHCNEVDPGAEVVPNECSVHEATPPSGNGPQKKSVDRSIQTVVSCLFNPENRLRNSVVTQDDYFKDKRVHHFRRSRAMLKSV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | deleted in azoospermia-like |
| 别名 | DAZH, DAZL1, DAZLA, DAZ-like autosomal, Deleted in azoospermia-like |
| 计算分子量 | 295 aa, 33 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC027595 |
| 基因名称 | DAZL |
| Gene ID (NCBI) | 1618 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q92904 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DAZL proteins are germ cell-specific RNA-binding proteins and are considered major regulators of spermatogenesis. Because DAZL is a novel protein expressed specifically in germ cells, it is widely employed as a germ cell marker. In humans, immunostaining shows DAZL is abundant in the cytoplasm of primary spermatocytes, but scarce in spermatogonia.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 DAZL antibody CL488-86344-3 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
