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验证数据展示

  • WB analysis using 86344-3-RR (same clone as 86344-3-PBS)

    WB analysis using 86344-3-RR (same clone as 86344-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86344-3-RR (DAZL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86344-3-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 86344-3-RR (same clone as 86344-3-PBS)

    IP experiment of mouse testis using 86344-3-RR (same clone as 86344-3-PBS)

    IP result of anti-DAZL (IP:86344-3-RR, 4ug; Detection:86344-3-RR 1:600) with mouse testis tissue lysate 1680 ug. This data was developed using the same antibody clone with 86344-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 86344-3-RR (same clone as 86344-3-PBS)

    IHC staining of mouse testis using 86344-3-RR (same clone as 86344-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86344-3-RR (DAZL antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86344-3-PBS in a different storage buffer formulation.

  • IHC staining of rat testis using 86344-3-RR (same clone as 86344-3-PBS)

    IHC staining of rat testis using 86344-3-RR (same clone as 86344-3-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 86344-3-RR (DAZL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86344-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 86344-3-RR (same clone as 86344-3-PBS)

    IHC staining of mouse testis using 86344-3-RR (same clone as 86344-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86344-3-RR (DAZL antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86344-3-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 86344-3-RR (same clone as 86344-3-PBS)

    IF Staining of mouse testis using 86344-3-RR (same clone as 86344-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DAZL antibody (86344-3-RR, Clone: 251035E11 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86344-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86344-3-RR

    Affinity and Kinetic Characterization of 86344-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86344-3-RR against Human DAZL were performed. The affinity constant is 2.52 nM.

DAZL Recombinant monoclonal antibody, PBS Only

DAZL Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 86344-3-PBS

产品说明书

CloneNo. 251035E11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, Indirect ELISA

别名

DAZH, DAZL1, DAZLA, DAZ-like autosomal, Deleted in azoospermia-like

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86344-3-PBS targets DAZL in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag37074

Product name: Recombinant human DAZL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 200-295 aa of BC027595

Sequence: QRSYVVPPAYSAVNYHCNEVDPGAEVVPNECSVHEATPPSGNGPQKKSVDRSIQTVVSCLFNPENRLRNSVVTQDDYFKDKRVHHFRRSRAMLKSV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 deleted in azoospermia-like
别名 DAZH, DAZL1, DAZLA, DAZ-like autosomal, Deleted in azoospermia-like
计算分子量 295 aa, 33 kDa
观测分子量37 kDa
GenBank蛋白编号BC027595
基因名称 DAZL
Gene ID (NCBI) 1618
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ92904
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DAZL proteins are germ cell-specific RNA-binding proteins and are considered major regulators of spermatogenesis. Because DAZL is a novel protein expressed specifically in germ cells, it is widely employed as a germ cell marker. In humans, immunostaining shows DAZL is abundant in the cytoplasm of primary spermatocytes, but scarce in spermatogonia.

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