• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 86344-3-RR

    WB analysis using 86344-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86344-3-RR (DAZL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 86344-3-RR

    IP experiment of mouse testis using 86344-3-RR

    IP result of anti-DAZL (IP:86344-3-RR, 4ug; Detection:86344-3-RR 1:600) with mouse testis tissue lysate 1680 ug.

  • IHC staining of mouse testis using 86344-3-RR

    IHC staining of mouse testis using 86344-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86344-3-RR (DAZL antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 86344-3-RR

    IHC staining of rat testis using 86344-3-RR

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 86344-3-RR (DAZL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 86344-3-RR

    IHC staining of mouse testis using 86344-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86344-3-RR (DAZL antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 86344-3-RR

    IF Staining of mouse testis using 86344-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DAZL antibody (86344-3-RR, Clone: 251035E11 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 86344-3-RR

    Affinity and Kinetic Characterization of 86344-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86344-3-RR against Human DAZL were performed. The affinity constant is 2.52 nM.

DAZL Recombinant monoclonal antibody

DAZL Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 86344-3-RR

产品说明书

CloneNo. 251035E11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, ELISA

别名

DAZH, DAZL1, DAZLA, DAZ-like autosomal, Deleted in azoospermia-like

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inhuman testis tissue, mouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86344-3-RR targets DAZL in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag37074

Product name: Recombinant human DAZL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 200-295 aa of BC027595

Sequence: QRSYVVPPAYSAVNYHCNEVDPGAEVVPNECSVHEATPPSGNGPQKKSVDRSIQTVVSCLFNPENRLRNSVVTQDDYFKDKRVHHFRRSRAMLKSV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 deleted in azoospermia-like
别名 DAZH, DAZL1, DAZLA, DAZ-like autosomal, Deleted in azoospermia-like
计算分子量 295 aa, 33 kDa
观测分子量37 kDa
GenBank蛋白编号BC027595
基因名称 DAZL
Gene ID (NCBI) 1618
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ92904
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DAZL proteins are germ cell-specific RNA-binding proteins and are considered major regulators of spermatogenesis. Because DAZL is a novel protein expressed specifically in germ cells, it is widely employed as a germ cell marker. In humans, immunostaining shows DAZL is abundant in the cytoplasm of primary spermatocytes, but scarce in spermatogonia.

实验方案

Product Specific Protocols
IF protocol for DAZL antibody 86344-3-RRDownload protocol
IHC protocol for DAZL antibody 86344-3-RRDownload protocol
IP protocol for DAZL antibody 86344-3-RRDownload protocol
WB protocol for DAZL antibody 86344-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...