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验证数据展示

  • WB analysis using 22524-1-AP (same clone as 22524-1-PBS)

    WB analysis using 22524-1-AP (same clone as 22524-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22524-1-AP (DAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

    IHC staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

    IHC staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

    IHC staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

    IF Staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DAT antibody (22524-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

    IP experiment of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

    IP result of anti-Dopamine Transporter/DAT (IP:22524-1-AP, 4ug; Detection:22524-1-AP 1:1000) with mouse brain tissue lysate 1720 ug. This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

    IF Staining of mouse brain using 22524-1-AP (same clone as 22524-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using Dopamine Transporter/DAT antibody (22524-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22524-1-PBS in a different storage buffer formulation.

Dopamine Transporter/DAT Polyclonal antibody, PBS Only

Dopamine Transporter/DAT Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA
Cat No. 22524-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

别名

2, SLC6A3, DA transporter, DAT, DAT1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

22524-1-PBS targets Dopamine Transporter/DAT in WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18297

Product name: Recombinant human SLC6A3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-62 aa of BC133003

Sequence: MSKSKCSVGLMSSVVAPAKEPNAVGPKEVELILVKEQNGVQLTSSTLTNPRQSPVEAQDRET

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
别名 2, SLC6A3, DA transporter, DAT, DAT1
计算分子量 620 aa, 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC133003
基因名称 DAT
Gene ID (NCBI) 6531
RRIDAB_2879116
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01959
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DAT, also name as SLC6A3, is dopamine transporter which is a member of the sodium- and chloride- dependent neurotransmitter transporter family. Dopamine(DA) released from neurons is cleared by the DA transporter (DAT). Altered dopaminergic signaling is linked to multiple neuropsychiatric disorders, such as attention deficit hyperactive disorder, mood disorders, schizophrenia, autism and so on (PMID:30755521). 22524-1-AP can detect 70~80kDa band, which is consistent with the report (PMID:12746456).

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