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验证数据展示

  • WB analysis using 30656-1-AP (same clone as 30656-1-PBS)

    WB analysis using 30656-1-AP (same clone as 30656-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30656-1-AP (DAPK3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30656-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 30656-1-AP (same clone as 30656-1-PBS)

    IHC staining of human lung cancer using 30656-1-AP (same clone as 30656-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30656-1-AP (DAPK3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30656-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DAPK3 antibody included
  • IF Staining of A431 using 30656-1-AP (same clone as 30656-1-PBS)

    IF Staining of A431 using 30656-1-AP (same clone as 30656-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DAPK3 antibody (30656-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30656-1-PBS in a different storage buffer formulation.

DAPK3 Polyclonal antibody, PBS Only

DAPK3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30656-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ZIP kinase, DAP kinase 3, DAP like kinase, DAP-like kinase, Death-associated protein kinase 3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy DAPK3 Ready-To-Use IHC Kit. DAPK3 primary antibody included.

产品信息

30656-1-PBS targets DAPK3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33051

Product name: Recombinant human DAPK3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 252-361 aa of NM_001348

Sequence: IRRLLVKDPKRRMTIAQSLEHSWIKAIRRRNVRGEDSGRKPERRRLKTTRLKEYTIKSHSSLPPNNSYADFERFSKVLEEAAAAEEGLRELQRSRRLCHEDVEALAAIYE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 death-associated protein kinase 3
别名 ZIP kinase, DAP kinase 3, DAP like kinase, DAP-like kinase, Death-associated protein kinase 3
计算分子量 53 kDa
观测分子量37, 52 kDa
GenBank蛋白编号NM_001348
基因名称 DAPK3
Gene ID (NCBI) 1613
RRIDAB_3086381
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43293
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DAPK3, also named as ZIPK and Dlk, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. The calculated molecular weight of DAPK3 is 52 kDa. This antibody can recognize the 37 kDa isoform of target. ZIP kinase is a Serine/threonine kinase which acts as a positive regulator of apoptosis. ZIP kinasephosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. ZIP kinase regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. It catalyze the reaction: ATP + a protein = ADP + a phosphoprotein.

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