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验证数据展示

  • WB analysis using 30656-1-AP

    WB analysis using 30656-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30656-1-AP (DAPK3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 30656-1-AP

    IF Staining of A431 using 30656-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DAPK3 antibody (30656-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

DAPK3 Polyclonal antibody

DAPK3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30656-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IF/ICC, ELISA and More (1)

别名

ZIP kinase, EC:2.7.11.1, Dlk, Death-associated protein kinase 3, DAP-like kinase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, Hela cells, HepG2 cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy DAPK3 Ready-To-Use IHC Kit. DAPK3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30656-1-AP targets DAPK3 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, CoIP
经测试反应性 human
文献引用反应性mouse
免疫原 DAPK3 fusion protein Ag33051 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 death-associated protein kinase 3
别名 ZIP kinase, EC:2.7.11.1, Dlk, Death-associated protein kinase 3, DAP-like kinase
计算分子量 53 kDa
观测分子量37, 52 kDa
GenBank蛋白编号NM_001348
基因名称 DAPK3
Gene ID (NCBI) 1613
RRIDAB_3086381
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43293
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DAPK3, also named as ZIPK and Dlk, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. The calculated molecular weight of DAPK3 is 52 kDa. This antibody can recognize the 37 kDa isoform of target. ZIP kinase is a Serine/threonine kinase which acts as a positive regulator of apoptosis. ZIP kinasephosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. ZIP kinase regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. It catalyze the reaction: ATP + a protein = ADP + a phosphoprotein.

实验方案

Product Specific Protocols
WB protocol for DAPK3 antibody 30656-1-APDownload protocol
IF protocol for DAPK3 antibody 30656-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,CoIP

Biochem Biophys Res Commun

Protection of blood-brain barrier by endothelial DAPK1 deletion after stroke

Authors - Zhijiang Gu
View Article