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验证数据展示

  • WB analysis using 20048-1-AP (same clone as 20048-1-PBS)

    WB analysis using 20048-1-AP (same clone as 20048-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 20048-1-AP (same clone as 20048-1-PBS)

    WB analysis of HeLa using 20048-1-AP (same clone as 20048-1-PBS)

    WB result of DAPK2-Specific antibody (20048-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DAPK2-Specific transfected HeLa cells. This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 20048-1-AP (same clone as 20048-1-PBS)

    IHC staining of mouse skeletal muscle using 20048-1-AP (same clone as 20048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 20048-1-AP (same clone as 20048-1-PBS)

    IHC staining of mouse skeletal muscle using 20048-1-AP (same clone as 20048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 20048-1-AP (same clone as 20048-1-PBS)

    IF Staining of HepG2 using 20048-1-AP (same clone as 20048-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DAPK2-Specific antibody (20048-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20048-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

DAPK2-Specific Polyclonal antibody, PBS Only

DAPK2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20048-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DAPK2, DAP kinase 2, DAP kinase related protein 1, DAP-kinase-related protein 1, Death-associated protein kinase 2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20048-1-PBS targets DAPK2-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 death-associated protein kinase 2
别名 DAPK2, DAP kinase 2, DAP kinase related protein 1, DAP-kinase-related protein 1, Death-associated protein kinase 2
计算分子量 43 kDa
观测分子量 38-43 kDa
GenBank蛋白编号NM_014326
基因名称 DAPK2
Gene ID (NCBI) 23604
RRIDAB_10667409
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UIK4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DAPK2, also named as DRP-1, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. DAPK2 is a calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK2 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to DAPK2.

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