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验证数据展示

  • WB analysis using 20048-1-AP

    WB analysis using 20048-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 20048-1-AP

    WB analysis of HeLa using 20048-1-AP

    WB result of DAPK2-Specific antibody (20048-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DAPK2-Specific transfected HeLa cells.

  • IHC staining of mouse skeletal muscle using 20048-1-AP

    IHC staining of mouse skeletal muscle using 20048-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 20048-1-AP

    IHC staining of mouse skeletal muscle using 20048-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A375 using 20048-1-AP

    IF Staining of A375 using 20048-1-AP

    Immunofluorescent analysis of A375 cells using 20048-1-AP (DAPK2-Specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

DAPK2-Specific Polyclonal antibody

DAPK2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20048-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

DAPK2, DAP kinase 2, DAP kinase related protein 1, DAP-kinase-related protein 1, Death-associated protein kinase 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, A431 cells, HepG2 cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20048-1-AP targets DAPK2-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 death-associated protein kinase 2
别名 DAPK2, DAP kinase 2, DAP kinase related protein 1, DAP-kinase-related protein 1, Death-associated protein kinase 2
计算分子量 43 kDa
观测分子量 38-43 kDa
GenBank蛋白编号NM_014326
基因名称 DAPK2
Gene ID (NCBI) 23604
RRIDAB_10667409
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UIK4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DAPK2, also named as DRP-1, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. DAPK2 is a calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK2 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to DAPK2.

实验方案

Product Specific Protocols
WB protocol for DAPK2-Specific antibody 20048-1-APDownload protocol
IHC protocol for DAPK2-Specific antibody 20048-1-APDownload protocol
IF protocol for DAPK2-Specific antibody 20048-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

J Cancer

Identification and validation of a novel apoptosis-related prognostic risk score model for lung adenocarcinoma

Authors - Yan Wang
View Article