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验证数据展示

  • FC experiment of HepG2 using 66364-1-Ig

    FC experiment of HepG2 using 66364-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human D2HGDH (66364-1-Ig, Clone:1E9B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • WB analysis of NIH/3T3 using 66364-1-Ig

    WB analysis of NIH/3T3 using 66364-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66364-1-Ig (D2HGDH Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66364-1-Ig

    IHC staining of human liver cancer using 66364-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66364-1-Ig (D2HGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66364-1-Ig

    IHC staining of human liver cancer using 66364-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66364-1-Ig (D2HGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 66364-1-Ig

    IF Staining of HepG2 using 66364-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using D2HGDH antibody (66364-1-Ig, Clone: 1E9B3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

D2HGDH Monoclonal antibody

D2HGDH Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66364-1-Ig

产品说明书

CloneNo. 1E9B3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

1E9B3, D2HGD, D-2-hydroxyglutarate dehydrogenase, mitochondrial, EC:1.1.99.39

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, C6 cells, rat heart tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66364-1-Ig targets D2HGDH in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5036

Product name: Recombinant human D2HGDH protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-286 aa of BC036604

Sequence: MLPRRPLAWPAWLLRGAPGAAGSWGRPVGPLARRGCCSAPGTPEVPLTRERYPVQRLPFSTVSKQDLAAFERIVPGGVVTDPEALQAPNVDWLRTLRGCSKVLLRPRTSEEVSHILRHCHERNLAVNPQGGNTGMVGGSVPVFDEIILSTARMNRVLSFHSVSGILVCQAGCVLEELSRYVEERDFIMPLDLGAKGSCHIGGNVATNAGGLRFLRYGSLHGTVLGLEVVLADGTVLDCLTSLRKDNTGYDLKQLFIGSEGTLGIITTVSILCPPKPRAVNVAFLGC

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 D-2-hydroxyglutarate dehydrogenase
别名 1E9B3, D2HGD, D-2-hydroxyglutarate dehydrogenase, mitochondrial, EC:1.1.99.39
计算分子量 521 aa, 56 kDa
观测分子量 56 kDa
GenBank蛋白编号BC036604
基因名称 D2HGDH
Gene ID (NCBI) 728294
RRIDAB_2881744
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8N465
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

D2HGDH(D-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as D2HGD and belongs to the FAD-binding oxidoreductase/transferase type 4 family.It catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.Defects in D2HGDH are the cause of D-2-hydroxyglutaric aciduria type 1 (D2HGA1).

实验方案

Product Specific Protocols
FC protocol for D2HGDH antibody 66364-1-IgDownload protocol
IF protocol for D2HGDH antibody 66364-1-IgDownload protocol
IHC protocol for D2HGDH antibody 66364-1-IgDownload protocol
WB protocol for D2HGDH antibody 66364-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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