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验证数据展示

  • FC experiment of HeLa using CL488-10384

    FC experiment of HeLa using CL488-10384

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Cytokeratin 8 (CL488-10384) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of MCF-7 using CL488-10384

    IF Staining of MCF-7 using CL488-10384

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 Cytokeratin 8 antibody (CL488-10384) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Cytokeratin 8 Polyclonal antibody

Cytokeratin 8 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10384

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

KRT8, CARD2, CK 8, CK8, CK-8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CK8 Ready-To-Use IHC Kit. CK8 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10384-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10384 targets Cytokeratin 8 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Cytokeratin 8 fusion protein Ag0488 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 8
别名 KRT8, CARD2, CK 8, CK8, CK-8
计算分子量 54 kDa
观测分子量 52 kDa
GenBank蛋白编号BC000654
基因名称 Cytokeratin 8
Gene ID (NCBI) 3856
RRIDAB_3083875
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05787
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 8 antibody CL488-10384Download protocol
FC protocol for CL Plus 488 Cytokeratin 8 antibody CL488-10384Download protocol
Standard Protocols
Click here to view our Standard Protocols