验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85423-2-RR targets Cytokeratin 6B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Cytokeratin 6B fusion protein Ag11288 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | keratin 6B |
| 别名 | KRT6B, 242874F9, CK 6B, CK5/6, CK6B |
| 计算分子量 | 564 aa, 60 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC034535 |
| 基因名称 | Cytokeratin 6B |
| Gene ID (NCBI) | 3854 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04259 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cytokeratin 6B (CK6B) is a type of intermediate filament protein primarily expressed in certain epithelial cells. Cytokeratin 6B consists of a central α-helical rod domain flanked by non-helical head and tail domains, which is a common structural feature of cytokeratins. CK6B is mainly expressed in the basal and suprabasal layers of stratified epithelia, such as the oral mucosa, esophagus, and conjunctiva. It is also found in some specialized epithelial cells like the hair follicle outer root sheath and the nail matrix. CK6B can serve as a diagnostic marker for certain pathological conditions. Its expression pattern can be used to distinguish between different types of epithelial tumors or to identify specific epithelial cell populations in histopathological studies.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 6B antibody 85423-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |