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验证数据展示

  • WB analysis of A431 using 16572-1-AP (same clone as 16572-1-PBS)

    WB analysis of A431 using 16572-1-AP (same clone as 16572-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 16572-1-AP (Cytokeratin 4 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 16572-1-AP (same clone as 16572-1-PBS)

    IHC staining of human cervical cancer using 16572-1-AP (same clone as 16572-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16572-1-AP (Cytokeratin 4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 16572-1-AP (same clone as 16572-1-PBS)

    IHC staining of human cervical cancer using 16572-1-AP (same clone as 16572-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16572-1-AP (Cytokeratin 4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 16572-1-AP (same clone as 16572-1-PBS)

    IF Staining of A431 using 16572-1-AP (same clone as 16572-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 4 antibody (16572-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse skin using 16572-1-AP (same clone as 16572-1-PBS)

    IF Staining of mouse skin using 16572-1-AP (same clone as 16572-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using Cytokeratin 4 antibody (16572-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16572-1-PBS in a different storage buffer formulation.

Cytokeratin 4 Polyclonal antibody, PBS Only

Cytokeratin 4 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 16572-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16572-1-PBS targets Cytokeratin 4 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag9854

Product name: Recombinant human KRT4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 184-534 aa of BC042174

Sequence: NLLQQQTTTTSSKNLEPLFETYLSVLRKQLDTLGNDKGRLQSELKTMQDSVEDFKTKYEEEINKRTAAENDFVVLKKDVDAAYLNKVELEAKVDSLNDEINFLKVLYDAELSQMQTHVSDTSVVLSMDNNRNLDLDSIIAEVRAQYEEIAQRSKAEAEALYQTKVQQLQISVDQHGDNLKNTKSEIAELNRMIQRLRAEIENIKKQCQTLQVSVADAEQRGENALKDAHSKRVELEAALQQAKEELARMLREYQELMSVKLALDIEIATYRKLLEGEEYRMSGECQSAVSISVVSGSTSTGGISGGLGSGSGFGLSSGFGSGSGSGFGFGGSVSGSSSSKIISTTTLNKRR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 4
别名
计算分子量 534 aa, 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC042174
基因名称 Cytokeratin 4
Gene ID (NCBI) 3851
RRIDAB_2134041
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP19013
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 4 is a type II cytokeratins. It is specifically found in differentiated layers of the mucosal and esophageal epithelia together with keratin 13.

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