Cytokeratin 4 Polyclonal antibody
Cytokeratin 4 Polyclonal Antibody for FC, IF/ICC,IF-P, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
FC, IF/ICC,IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, human bladder tissue |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | A431 cells, mouse skin tissue |
| Positive FC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16572-1-AP targets Cytokeratin 4 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | FC, IF/ICC,IF-P, IHC, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | Cytokeratin 4 fusion protein Ag9854 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | keratin 4 |
| Synonyms | CK 4, CK4, CYK4, Cytokeratin 4, K4, keratin 4, KRT4, Type II keratin Kb4 |
| Calculated Molecular Weight | 534 aa, 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC042174 |
| Gene Symbol | KRT4 |
| Gene ID (NCBI) | 3851 |
| RRID | AB_2134041 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P19013 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 4 is a type II cytokeratins. It is specifically found in differentiated layers of the mucosal and esophageal epithelia together with keratin 13.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 4 antibody 16572-1-AP | Download protocol |
| IHC protocol for Cytokeratin 4 antibody 16572-1-AP | Download protocol |
| IF protocol for Cytokeratin 4 antibody 16572-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature A single-cell atlas of the airway epithelium reveals the CFTR-rich pulmonary ionocyte. | ||
Development Novel dynamics of human mucociliary differentiation revealed by single-cell RNA sequencing of nasal epithelial cultures. | ||
Int J Mol Sci Early Clinical Outcomes of the First Commercialized Human Autologous Ex Vivo Cultivated Oral Mucosal Epithelial Cell Transplantation for Limbal Stem Cell Deficiency: Two Case Reports and Literature Review | ||
Ann Diagn Pathol The molecular-based differentiation of Heck's disease from its mimics including oral condyloma and white sponge nevus. | ||
Cell Rep An Immunocompetent Mouse Model of HPV16(+) Head and Neck Squamous Cell Carcinoma. | ||
Oncotarget Significance of the NOR1-FOXA1/HDAC2-Slug regulatory network in epithelial-mesenchymal transition of tumor cells. |
